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Product Specification for Alkaline
Phosphatase
Orthophosphoric-monoester phosphohydrolase
(alkaline optimum)
EC 3.1.3.1
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Alkaline phosphatase |
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Orthophosphate monoester + H2O
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Alcohol + Orthophosphate |
Availability
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PRODUCT
CODES |
Specification |
01001 |
01005 |
01006 |
AVAILABLE SHORTLY
01003 |
| Form |
A freeze-dried powder |
A freeze-dried powder |
A freeze-dried powder |
Available in
glycerol, TEA buffer or ammonium sulphate. |
| Activity |
> 20 u/mg material |
> 50 u/mg material |
± 100 u/mg material |
>4000 u/mg protein |
| Storage |
For optimum retention of
activity, the freeze-dried product should be stored at -10°C or lower and the
liquid preparations should be stored refrigerated at 4°C. |
| Unit Definition |
One unit of activity is defined as that
amount of enzyme which causes the hydrolysis of one micromole of p-nitrophenyl phosphate
per minute at pH 9.8 and 37°C (diethanolamine buffer) |
| Unit Conversion |
One glycine unit is
equivalent to approximately three DEA units where the glycine unit is defined as:
That amount of enzyme causing the hydrolysis of one micromole of p-nitrophenyl phosphate
per minute at pH 9.6 and 25°C in glycine buffer. |
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Specialists in the Isolation and Purification of Horseradish
Peroxidase and a Select Range of Enzymes