Specialists in the Isolation and Purification of Horseradish Peroxidase and a Select Range of Enzymes

Assay Procedure (Ref. No. FGAP057) - Chymotrypsinogen

To determine the potential activity of the inactive zymogen, a solution of the preparation must be activated and the activity then measured by the method of assay for chymotrypsin.

The method of assay is that suggested by Schwert and Takenaka in which N-acetyl-L-tyrosine ethyl ester (ATEE) is hydrolyzed at the ester linkage causing a decrease of absorbance measured at 237 nm and 25^°C.

That amount of activated zymogen causing a decrease in absorbance at 237nm of 0.0075 per minute at 25^°C resulting from the hydrolysis of ATEE under the specified conditions.

Procedure

Refer to Assay Procedure Ref. No. FGAP034 for Chymotrypsin.

Please contact us should you require details of a suitable procedure for the activation of chymotrypsinogen to chymotrypsin.

Native activity is determined by measuring activity of chymotrypsin in chymotrypsinogen.

Schwert G.W., and Takenaka Y.: (1955) Biochim. Biophys. Acta 16 570.